Free research tool

Peptide Reconstitution Calculator

Enter the lyophilized amount in your vial, the volume of bacteriostatic water you add, and a target analyte mass. The calculator returns the solution concentration, the aliquot volume in milliliters and microliters, and how many aliquots a vial yields. For laboratory reconstitution calculations only.

Lyophilized peptide per vial (mg)Bacteriostatic water added (mL)

Target analyte mass per aliquot

Solution concentration2.5 mg/mL

Aliquot volume0.1 mL

Aliquot volume (µL)100 µL

Aliquots per vial≈ 20

For laboratory reconstitution calculations only. Not medical, dosing, or clinical guidance, and not instructions for administration.

How reconstitution math works

Lyophilized peptides ship as a freeze-dried powder and must be dissolved in a diluent — typically bacteriostatic water — before use in research. The resulting concentration is simply the peptide mass divided by the diluent volume: a 5 mg vial reconstituted with 2 mL of bacteriostatic water yields 2.5 mg/mL (2,500 mcg/mL). The volume containing a target analyte mass is that mass divided by the concentration: 250 mcg ÷ 2,500 mcg/mL = 0.1 mL (100 µL), which a pipette measures directly.

Adding more diluent makes pipetting easier (each aliquot is a larger, more precisely measurable volume) while adding less keeps aliquot volumes small. The total peptide in the vial never changes — only the concentration does.

Reconstitution & storage quick reference

Use bacteriostatic water for solutions held over multiple sessions — the 0.9% benzyl alcohol inhibits bacterial growth. Sterile water suits single-use preparation only.
Direct the diluent stream down the vial wall, not onto the lyophilized cake, and swirl gently — never shake — to preserve peptide integrity.
Store lyophilized vials at −20°C long-term (2–8°C short-term), protected from light. Reconstituted solutions are typically held at 2–8°C and used within the window appropriate to the compound under study.
Minimize freeze–thaw cycles; aliquot if a study design requires repeated access.

All Eon Research compounds ship lyophilized in multi-vial research kits from our US facility with tracking. This tool and the information above are provided for in-vitro laboratory research calculations only and do not constitute medical, dosing, or clinical guidance.