MOTS-c Reconstitution Calculator

There is no single required volume — the diluent sets the concentration, not the amount of MOTS-c in the vial. A 10mg vial (10 mg) gives 10 mg/mL with 1 mL of bacteriostatic water, 5 mg/mL with 2 mL, and 3.33 mg/mL with 3 mL. More diluent makes each aliquot a larger, more precisely measurable volume.

Concentration is the lyophilized mass divided by the diluent volume. A 40mg MOTS-c vial reconstituted with 2 mL of bacteriostatic water is 20 mg/mL (20,000 mcg/mL). MOTS-c is supplied in 10mg, 40mg vials; the chart on this page lists each size at 1, 2 and 3 mL.

A U-100 graduated syringe has 100 marks per milliliter, so each mark is 0.01 mL. At 5 mg/mL (a 10mg vial with 2 mL) each mark holds 50 mcg; multiply the mcg-per-mark figure by the number of marks to find the mass in a measured volume. These are volume-measurement figures, not dosing or administration instructions.

Lyophilized MOTS-c is stored at −20°C long-term (2–8°C short-term), protected from light. Once reconstituted with bacteriostatic water, the solution is typically held at 2–8°C and used within the window appropriate to the study. Swirl gently to dissolve — never shake — and minimize freeze–thaw cycles.

No. It performs in-vitro laboratory reconstitution math for a research-use-only reference standard. It is not medical, dosing, or clinical guidance and is not instructions for administration.