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Free research tool · Cognitive & Neuro

Semax Reconstitution Calculator

Also listed as Semax peptide · ACTH 4-10 analog · N-acetyl semax

Semax ships lyophilized in 5mg, 10mg, 30mg vials. Enter the vial size, the bacteriostatic water you add, and a target analyte mass to get the solution concentration, the aliquot volume in mL and µL, and the marks it reaches on a U-100 graduated syringe. Laboratory reconstitution math only.

Target analyte mass per aliquot
Graduated syringe scale

U-100 scale — 100 graduation marks per mL.

Result rounding
Draw to (on a 1 mL syringe)10 units
0u50u100u
Aliquot volume0.1 mL · 100 µL
Solution concentration2.5 mg/mL
Concentration2,500 mcg/mL
Mass per syringe unit25 mcg / unit
Aliquots per vial≈ 20
Total per vial5,000 mcg

For laboratory reconstitution calculations only. Not medical, dosing, or clinical guidance, and not instructions for administration.

Semax reconstitution chart

Example: a 5mg Semax vial reconstituted with 2 mL of bacteriostatic water is 2.5 mg/mL (2,500 mcg/mL), and each mark on a U-100 graduated syringe holds 25 mcg. Every vial size and diluent volume:

Semax concentration by vial size and bacteriostatic water volume
VialBac watermg/mLmcg/mLmcg per U-100 mark
5mg1 mL55,00050
5mg2 mL2.52,50025
5mg3 mL1.671,66716.67
10mg1 mL1010,000100
10mg2 mL55,00050
10mg3 mL3.333,33333.33
30mg1 mL3030,000300
30mg2 mL1515,000150
30mg3 mL1010,000100

About Semax

ACTH(4-7) analog studied in neuroprotection, BDNF-signaling and cognition research models.

CAS number
80714-61-0
Form
Lyophilized powder

Semax calculator — frequently asked questions

There is no single required volume — the diluent sets the concentration, not the amount of Semax in the vial. A 5mg vial (5 mg) gives 5 mg/mL with 1 mL of bacteriostatic water, 2.5 mg/mL with 2 mL, and 1.67 mg/mL with 3 mL. More diluent makes each aliquot a larger, more precisely measurable volume.

Concentration is the lyophilized mass divided by the diluent volume. A 30mg Semax vial reconstituted with 2 mL of bacteriostatic water is 15 mg/mL (15,000 mcg/mL). Semax is supplied in 5mg, 10mg, 30mg vials; the chart on this page lists each size at 1, 2 and 3 mL.

A U-100 graduated syringe has 100 marks per milliliter, so each mark is 0.01 mL. At 2.5 mg/mL (a 5mg vial with 2 mL) each mark holds 25 mcg; multiply the mcg-per-mark figure by the number of marks to find the mass in a measured volume. These are volume-measurement figures, not dosing or administration instructions.

Lyophilized Semax is stored at −20°C long-term (2–8°C short-term), protected from light. Once reconstituted with bacteriostatic water, the solution is typically held at 2–8°C and used within the window appropriate to the study. Swirl gently to dissolve — never shake — and minimize freeze–thaw cycles.

No. It performs in-vitro laboratory reconstitution math for a research-use-only reference standard. It is not medical, dosing, or clinical guidance and is not instructions for administration.

More cognitive & neuro calculators

Semax is sold as a research-use-only reference standard in multi-vial kits shipped from our US facility with tracking. This tool and the information above are provided for in-vitro laboratory research calculations only and do not constitute medical, dosing, or clinical guidance.